![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
3
Part:BBa_K316010:Design
Designed by: IC 2010 Team Group: iGEM10_Imperial_College_London (2010-10-22)
Reverse strand coding XylE under LacI activation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1076
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 437
Illegal NgoMIV site found at 609
Illegal AgeI site found at 86 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
To obtain a 3' coding segment a number of PRC steps were required, for details please see our wiki[http://2010.igem.org/Team:Imperial_College_London/Strategy]
Source
Biobricks BBa_K143000 and BBa_J33204.