Composite
3

Part:BBa_K316010:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-22)

Reverse strand coding XylE under LacI activation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1076
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 437
    Illegal NgoMIV site found at 609
    Illegal AgeI site found at 86
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To obtain a 3' coding segment a number of PRC steps were required, for details please see our wiki[http://2010.igem.org/Team:Imperial_College_London/Strategy]

Source

Biobricks BBa_K143000 and BBa_J33204.